Investigating Cancer Tumor Microenvironment In vitro: Co-Culture Studies on Adipocytes and Cancer Cells


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Rencüzoğulları Ö., Özfiliz Kılbaş P., Bal E., Ayhan Şahin B.

in: Technologies in Cell Culture-A Journey From Basics to Advanced Applications , Prof. Soumya Basu,Dr. Amit Ranjan,Dr. Subhayan Sur, Editor, IntechOpen, London, pp.1-21, 2023

  • Publication Type: Book Chapter / Chapter Other Book
  • Publication Date: 2023
  • Publisher: IntechOpen
  • City: London
  • Page Numbers: pp.1-21
  • Editors: Prof. Soumya Basu,Dr. Amit Ranjan,Dr. Subhayan Sur, Editor
  • Istanbul Kültür University Affiliated: Yes

Abstract

The tumor microenvironment increases the growth and invasion of cancer cells, makes classical chemotherapy applications inadequate, and is associated with a poor cancer prognosis. Recent studies reveal that cancer stroma supports tumor growth and metastasis and develops resistance to chemotherapy. In vitro co-culture techniques are widely used to study cross-talk between tumor microenvironment cells such as adipocytes, endothelial cells, fibroblasts, macrophages, and cancer cells. Co-culture techniques are classified into two main categories: indirect and direct methods. Transwell (indirect) co-culture of mature adipocytes with cancer cells has shown cancer cell viability, growth, proliferation, invasion, and metastases. This chapter covers the general methods of co-culture studies and will emphasize the results obtained on the co-culture of adipocytes and cancer cells.