Research Information System
in: Technologies in Cell Culture-A Journey From Basics to Advanced Applications , Prof. Soumya Basu,Dr. Amit Ranjan,Dr. Subhayan Sur, Editor, IntechOpen, London, pp.1-21, 2023
The tumor microenvironment increases the growth and invasion of cancer cells, makes classical chemotherapy applications inadequate, and is associated with a poor cancer prognosis. Recent studies reveal that cancer stroma supports tumor growth and metastasis and develops resistance to chemotherapy. In vitro co-culture techniques are widely used to study cross-talk between tumor microenvironment cells such as adipocytes, endothelial cells, fibroblasts, macrophages, and cancer cells. Co-culture techniques are classified into two main categories: indirect and direct methods. Transwell (indirect) co-culture of mature adipocytes with cancer cells has shown cancer cell viability, growth, proliferation, invasion, and metastases. This chapter covers the general methods of co-culture studies and will emphasize the results obtained on the co-culture of adipocytes and cancer cells.