Random amplified polymorphic DNA analysis of salt-tolerant tobacco mutants generated by gamma radiation


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Celik O., Atak C.

GENETICS AND MOLECULAR RESEARCH, cilt.14, sa.1, ss.1324-1337, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.4238/2015.february.13.12
  • Dergi Adı: GENETICS AND MOLECULAR RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1324-1337
  • İstanbul Kültür Üniversitesi Adresli: Evet

Özet

Salinity is one of the major problems limiting the yield of agricultural products. Radiation mutagenesis is used to improve salt-tolerant mutant plants. In this study, we aimed to improve salt-tolerant mutants of two oriental tobacco varieties. One thousand seeds of each variety (M-0) were irradiated with 100, 200, 300, and 400 Gy gamma rays by Cs-137 gamma. In the M-1 generation, 2999 single plants were harvested. The next season, these seeds were bulked and planted to obtain M-2 progeny. The seeds of 1900 M-2 plants were picked separately. Salinity tolerance was tested in the M-3 generation. Among M-3 plantlets, 10 salt-tolerant tobacco mutants were selected. According to the results of the selection studies, 100- and 200-Gy gamma radiation doses were the effective doses to obtain the desired mutants. Glutathione reductase enzyme activities of salt-tolerant tobacco mutants were determined biochemically as a stress-tolerance marker. The differences between control and salt-tolerant mutants belonging to the Akhisar 97 and Izmir Ozbas tobacco varieties were evaluated by random amplified polymorphic DNA analysis. The total polymorphism rate was 73.91%.