Analysis of Expressed Genes in Normal and Tumoral Mammary Gland Tissue of the Terrier Dog

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OZDEMIR OZGENTURK N., OMEROGLU ULU Z., Ulu S., ÇELİK M., TELLİOĞLU B., YILDIRIM F., ...Daha Fazla

Kafkas Üniversitesi Veteriner Fakültesi Dergisi, cilt.24, sa.3, ss.357-364, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 3
  • Basım Tarihi: 2018
  • Doi Numarası: 10.9775/kvfd.2017.18964
  • Dergi Adı: Kafkas Üniversitesi Veteriner Fakültesi Dergisi
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.357-364
  • İstanbul Kültür Üniversitesi Adresli: Evet

Özet

Mammary gland tumor is the most common type of tumor in female dogs. Data on genes that are involved in tumorigenesis and mechanismof tumor development are insufficient. Comparative studies have been conducted in order to see if tumorigenesis studies in the dog couldbe a model for human mammary gland tumors. In this study, we constructed two different cDNA libraries from mammary tissue, whichwere collected from a normal mammary tissue of a healthy Terrier dog and a tumoral mammary tissue of a sick dog. A total 2304 colonieswhich are randomly picked out from the two libraries were sequenced for developing a dog mammary gland ESTs collection. Raw EST datawere analyzed with Phred/Phrap programs and readable EST sequences were assembled with the CAP3 program. All of EST sequences weregrouped into 45 contig and 2203 singletons. Putative functions of all unique sequences were designated by NCBI BLAST based on genehomology and annotated by BLAST2GO. The results of this study are a very valuable resource for functional genome studies of the dogs.