Akademik Veri Yönetim Sistemi
8th International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, 17 - 19 Aralık 2025, sa.755, ss.264, (Özet Bildiri)
Drought and salinity are major abiotic stresses that significantly limit plant growth and productivity. These stresses are increasing in severity their arable lands, particularly in areas with low rainfall and high evaporation, due to the effects of climate change. Sustainable agriculture, breeding and molecular approaches are essential to develop crop varieties tolerant to abiotic stresses. Comparative transcriptomic analyses provide valuable information about the mechanisms associated with stress response. This study used RNA-seq data generated from the SM1 and SM3-1 mutants, developed from the original soybean variety S04-05 by gamma irradiation. Drought and salinity tolerant mutants, were evaluated to identify transcriptional changes and single nucleotide variations (SNPs) associated with stress tolerance. Read quality was assessed with FASTQC (v0.12.1) and low-quality reads were removed with Trimmomatic (v0.40). High-quality reads were aligned to the soybean Williams 82 reference genome (Wm82 v2.a1) using the STAR tool. SNP calling was performed using GATK (v4.6.2.0). 22,549 SNPs in S04-05, 32,105 in SM1, and 27,273 in SM3-1 were identified. Comparative analyses identified 10,070 SNPs specific to SM1, 6,418 specific to SM3-1, and 12,071 common to both mutants. Unique SNPs across mutants were annotated using Ensembl Variant Effect Predictor (VEP). These SNPs were found to affect 13,534 genes in SM1 and 12,472 genes in SM3-1. The 12,071 common SNPs affected 7,347 genes in both mutants. Comparative SNP analysis revealed distinct clusters of variants in SM1 and SM3-1, particularly in genes involved in abiotic stress perception, energy metabolism, and signaling. Genes located in these enriched pathways showed both increased SNP density and significant expression changes. This study provides a comprehensive perspective by examining the relationship between SNP analyses based on RNA-seq data and observed stress tolerance in mutants. The results demonstrate that accumulated point mutations in the genome can lead to diverse, potentially biologically meaningful alterations of stress-related pathways.